The course focuses on the concentration, recovery, and isolation of biological molecules relevant in biotechnology. The characteristics of biological molecules such as proteins and biological fluids such as blood, fermentation, and cell culture broth, are discussed. The principles, advantages, and limitations of centrifugation, membranes, cell-disruption, two-phase extraction, precipitation crystallization, and electrical processes are discussed. Integrated bioseparation schemes are presented and many specific applications are discussed in detail.
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