This course describes how enzymes in cells and immobilized within matrices are valuable tools to prepare natural and non-natural molecules used in a wide range of applications such as commodity chemicals, sweeteners, polymers, nutraceuticals, drugs, tissue engineering matrices, surfactants, antimicrobials, coatings, and advanced materials. Furthermore, biocatalysis has become a primary tool to develop sustainable pathways for chemical manufacturing. Students are introduced to basic biochemical principles of enzymes and whole cell systems. Lectures on free enzymes discuss their catalytic mechanism(s), approaches to enable their practical use under non-aqueous conditions (e.g., immobilization on solid supports), effects of reaction media polarity and water content. The importance of protein and metabolic engineering to developing practical biocatalysts is discussed. Integrated within the course are discussions of green chemistry and sustainability.
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